AgreenSkills session, year: 2nd session, 2012
Receiving laboratory: MICALIS Food microbiology and human health Jouy-en-Josas, France
Country of origin : Poland
Genetic engineering of lipid metabolism and substrate utilization for improving biolipid production by oleaginous yeast
The yeast Yarrowia lipolytica is currently the subject of numerous studies due to its ability to produce molecules of interest such as citric acid, erythritol and biofuels. The production of alternative fuels by the mean of microorganisms from non-food resources has become an important challenge to face the increasing demand and pricing, for this reasons, Y. lipolytica appears to be a promising microbial biofuel producer. The scientific purpose of the project is to discover and to understand the mechanisms of lipid biosynthesis and accumulation in Y. lipolytica. In addition, carbon source utilization remains an essential step for these processes; therefore, hexose transport into the cell occurring in this yeast is an additional objective of this project. In the yeast Saccharomyces cerevisiae glucose and fructose are uptaken by the same family of transporters (HXT1-17). Therefore, to identify the presence of similar transporters in Y. lipolytica or to determine specific ones for glucose and fructose uptake will provide insight into this phenomenon. It will be equally essential to examine the mechanisms controlling gene expression as well as the factors that influence the rate of transport.
My research interests are focused on the sugar metabolism in yeast, particularly in Yarrowia lipolytica. I started working with this yeast during my PhD studies at the Department of Biotechnology and Food Microbiology Wroclaw University of Environmental and Life Sciences, Poland. I acquired an extensive experience with cultures of Y. lipolytica in bioreactors for the production of molecules for industry i.e. production of citric acid and simultaneously production of invertase. Together with Prof. Malgorzata Robak I also worked with bioremediation of oils contaminated soil using the Y. lipolytica yeast. As a recipient of the Swiss Federal Scholarship I completed a significant part of my PhD in the Prof. Markus Aebi group at the Institute of Microbiology Swiss Federal Institute of Technology – ETH in Zurich, Switzerland. Additionally, during my studies at ETH I worked on another independent project focused on the characterization of glycans occurring in the mushroom Coprinopsis cinerea. The results of this project were published in the Journal of Biological Chemistry.
- Lazar Z*, Rossignol T*, Verbeke J, Crutz-Le Coq AM, Nicaud JM, Robak M, Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial application, Journal of Industrial Microbiology and Biotechnology, 2013 (*equal contribution)
- Rywińska A, Juszczyk P, Wojtatowicz M, Robak M, Lazar Z, Tomaszewska L, Rymowicz W, Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications, review, Biomass and Bioenergy, 2013, 48, 148-166
- Śnieżewski Ł, Walczak E, Lazar Z, Robak M, Low peptone dose as inductor of alkaline protease promoter used for invertase gene expression in Yarrowia lipolytica, Journal of Life Sciences, 2012, 6, 1100-1108
- Lazar Z, Walczak E, Robak M, Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC+transformants, Bioresource Technology, 2011, 102, 6982-6989
- Robak M, Boruczkowski T, Drożdż W, Lazar Z, Baranowska M, Prządo D, Steininger M, Charczuk Z, Application of the Yeasts Yarrowia lipolytica for in-situ Bioremediation of Soil Contaminated with Creosote Oil – A Case Study , Ochrona Środowiska, 2011, 33, 2
- Buser R*, Lazar Z*, Kaser S, Kunzler M, Aebi M: Identification, characterization and biosynthesis of a novel N-glycan modification in the fruiting body of the basidiomycete Coprinopsis cinerea, Journal of Biological Chemistry, 2010, 285(14), 10715-10723 (*equal contribution)
- Walczak E, Lazar Z, Mauesberger S, Robak M., Evaluation of Yarrowia lipolytica transformants growth on lactose, Acta Scientiarum Polonorum Biotechnologia, 2007, 4, 21-29
- Award of the Rector of Wroclaw University of Environmental and Life Sciences, for research work and publication, 2012
- Robak M, Lazar Z, Walczak E, The method of bioproducts obtaining using yeast Yarrowia lipolytica, Patent Application No. P391223, 19.05.2010