AgreenSkills session, year: 2nd session, 2013
Receiving laboratory: Biozentrum Campus Riedberg, Physiology and Genetics of Lower eukaryotes, Germany
Country of origin : France
Development of a new synthetic assimilation pathway for the assimilation of pentose sugars
Evolution has selected microorganisms optimized for using carbon sources like hexoses and pentoses to increase their biomass. Metabolic engineering focuses on redirect the carbon flux from the biomass production to the production of molecules with high added-value. The mobility project consists in designing a synthetic metabolic pathway to assimilate xylose in Saccharomyces cerevisiae to allow it to produce two carbons compounds of industrial interest: glycolic acid and ethylene glycol instead of more biomass. Then, S. cerevisiae will be designed to co-assimilate both xylose and glucose.
I hold an Msc in microbiology and a PhD in interaction between plants and microorganisms. During this period I worked on the role of nitric oxide in the nitrogen fixing symbiosis between Sinorhizobium meliloti and Medicago truncatula in Claude Bruand’s group in the laboratory of plant-microorganism interaction, granted by a “contrat jeune scientifique” from INRA. I’ve published during my PhD four articles in peer-reviewed journals and one patent.
Now, I am doing a post-doc, still financed by my contrat jeune scientific grant. I’m working on another thematic, synthetic biology in microorganism metabolism in Jean-Marie François’s group in the laboratory of system biology engineering and processes. I earn a grant for my project called Pentosys through the Toulouse white biotechnology. This project consists of the building of synthetic metabolic pathways for the assimilation of pentoses in microorganisms for the production of high value products. Until now, I’ve performed this work on Escherichia coli.
I will be spending 6 months in Eckhard Boles’s lab in Frankfurt, Germany, to pursue my post-doc project on yeast.
I wish to pursue my career in the field of synthetic biology.
– E. Meilhoc, P. Blanquet, Y. Cam & C.Bruand (2013) Control of NO level in rhizobium-legume root nodules: Not only a plant globin story. Plant. Signal Behav. 8:10 (epub)
– Cam Y., O. Pierre, E. Boncompagni, D. Herouart, E. Meilhoc & C. Bruand (2012) Nitric oxide (NO): a key player in the senescence of Medicago truncatula 1 root nodules. New phytologist 196(2):548-60
– del Giudice, J., Y. Cam, I. Damiani, F. Fung-Chat, E. Meilhoc, C. Bruand, R. Brouquisse, A. Puppo & A. Boscari, (2011) Nitric oxide is required for an optimal establishment of the Medicago truncatula-Sinorhizobium meliloti symbiosis. New Phytologist 191: 405-417.
– Meilhoc, E., Y. Cam, A. Skapski & C. Bruand, (2010) The Response to Nitric Oxide of the Nitrogen-Fixing Symbiont Sinorhizobium meliloti. Molecular Plant-Microbe Interactions 23: 748-759.
Bruand C., Y. Cam, E. Meilhoc. A method for increasing legume productivity by cultivating a plant with an associated rhizobium overexpressing a flavohemoglobin protein. Ref: 359920D28984. International patent.