Vítor da Silveira Falavigna

Vítor da Silveira Falavigna
country
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AgreenSkills session, year: 2nd session, 2016

Receiving laboratory: AGAP Genetic Improvement and Plant Adaptation, Montpellier

Country of origin : Brazil

E-Mail: vitorfalavigna@gmail.com; vitor.falavigna@inra.fr

 

 

Mobility project

Deciphering the genetics and molecular control of bud dormancy in apple

Dormancy is an adaptive mechanism that enables perennial trees to survive unfavorable climatic conditions. Their regulatory mechanisms are highly heritable, suggesting a strong genetic control of this trait. Unlike other temperate fruit crops, dormancy in apple (Malus x domestica Borkh.) is triggered by exposure to low temperatures and not photoperiodic changes. Therefore, the predicted impact of the ongoing climate change will result in difficulties for apple production. At the molecular level, bud dormancy is probably controlled by a group of genes encoding MADS-box transcription factors commonly known as Dormancy-Associated MADS-box (DAM) genes. However, their precise mode of action and integration in gene networks controlling dormancy progression are still unknown. In this context, the present work aims to prospect and characterize the role of DAM and other flowering-time related genes in the dormancy process of apple through complementary genetic and molecular approaches. At the genetic level, genotyping-by-sequencing targeted approach on key gene families involved in dormancy and flowering regulation will be employed in an apple core collection established in France in order to identify allelic variation present in genes involved in bud dormancy and flowering control. At the molecular level, yeast two-hybrid and chromatin immunoprecipitation followed by sequencing (ChIP-seq) assays will be performed to identify how DAM proteins are organized in transcriptional complexes and also identify their genome-wide transcriptional targets, respectively. Together, these studies will allow a better characterization of key processes in dormancy molecular control, as well as to identify possible biotechnological resources for application in breeding programs.

Biography & research interests

I started to work as a trainee at the Laboratory of Plant Molecular Genetics of Embrapa Uva e Vinho – a federal research institute from Brazil – when I was an undergrad student. Further, I kept improving my qualifications by obtaining a Master and PhD titles in Cell and Molecular Biology at the Federal University of Rio Grande do Sul, Brazil. Nowadays, I am an AgreenSkills+ fellow at the Institut National de la Recherche Agronomique (INRA) at Montpellier, France. These experiences made me work in different fields and helped the development of a broad knowledge on plant biology, especially the ones involving genetic and molecular assays.

Selected publications

DA SILVA*, D.C.; FALAVIGNA*, V.S.; FASOLI, M.; BUFFON, V.; PORTO, D.D.; PAPPAS JR, G.J.; PEZZOTTI, M.; PASQUALI, G.; REVERS, L.F. Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. Horticulture Research, 3: 16042, 2016.
* These authors contributed equally to this work.

PORTO, D.D.; FALAVIGNA, V.S.; ARENHART, R.A.; PERINI, P.; BUFFON, V.; ANZANELLO, R.; SANTOS, H.P.; FIALHO, F.B.; OLIVEIRA, P.R.D.; REVERS, L.F. Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple. Tree Genetics & Genomes, 12: 46, 2016.

(*) FALAVIGNA, V.S.; MIOTTO, Y.E.; PORTO, D.D.; ANZANELLO, R.; SANTOS, H.P.; FIALHO, F.B.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L.F. Functional diversification of the dehydrin gene family in apple and its contribution to cold acclimation during dormancy. Physiologia Plantarum (Kobenhavn. 1948), 155: 315-329, 2015.

FALAVIGNA*, V.S.; PORTO*, D.D.; SILVEIRA, C.P.; REVERS, L.F. Recent advances in genetics and molecular control of bud dormancy in pipfruits. In: Anderson, J.V. Advances in Plant Dormancy. New York: Springer International Publishing, Chapter 5, p. 107-122, 2015.
* These authors contributed equally to this work.

(*) FALAVIGNA*, V.S.; PORTO*, D.D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L.F. Differential transcriptional profiles of dormancy-related genes in apple buds. Plant Molecular Biology Reporter, 32: 796-813, 2014.
* These authors contributed equally to this work.

Contact:

E-Mail: vitorfalavigna@gmail.com; vitor.falavigna@inra.fr

Website(s): http://umr-agap.cirad.fr/ | http://umr-agap.cirad.fr/equipes-scientifiques/architecture-et-fonctionnement-des-especes-fruitieres/liste-des-agents

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