AgreenSkills session, year: 1st session, 2012
Receiving laboratory: N&D Neurobiology and Development Jouy-en-Josas, France
Country of origin : Spain
Effect of long chain polyunsaturated fatty acids on neural stem cells in zebrafish (Danio rerio)
Adult neural stem cells (NSC) are scarce in humans, but other vertebrates contain several populations of continuously proliferating cells in their brain, which could be used as models to study post-embryonic neurogenesis. Indeed, neurogenesis has been extensively studied in the adults of several teleost fish species and previous research has described multiple neurogenic proliferating cell populations in the anterior central nervous system. An interesting cell population can be found in the optic tectum (OT), since previous BrdU and [3H] thymidine incorporation experiments both in zebrafish (Danio rerio) and medaka (Oryzias latipes) have shown the presence of proliferating cells in the OT. Studies on brain lipids have demonstrated that many lipids, the so-called “bioactive lipids”, have critical cell signalling functions. Different studies have shown that lipids act also through a new mechanism, which invokes lipid second messengers as regulators for the energy and redox balance of differentiating NSC. They are also critical for cell fate decisions during stem cell differentiation by inducing apoptosis or sustaining cell survival and polarity. Besides, essential fatty acids have a great relevance in brain health. Since cell membranes are responsible for receiving information and anticipating a response to any factor that perturbs the intracellular organization, changes in their fatty acid composition will directly affect cell function. The aim of this project is to study the effect of LC-PUFA on NSC of OT in vitro and in vivo considering that comparative studies of NSC biology would contribute to the understanding of these cells functioning.
In 2011 I concluded my PhD on the effect of long chain polyunsaturated fatty acids (LCPUFA) on neural activity related with fish larval behaviour in different marine at the University of Las Palmas de Gran Canaria, Spain. I developed behavioural and neural techniques applied to fish welfare getting high knowledge of fish nutrition in relation with fish health. During this time I was working in collaboration with different research groups such as the Institute of Neurobiology of Castilla y León (Spain) and the Neurobiology and Behaviour Department at Cornell University (USA). During this time, I studied the effect of LCPUFA on the structure of Mauthner cells in intact living fish larvae by injecting fluorescent dyes as a labelling method. I have worked in imaging analysis using modern optical techniques, such as confocal microscopy on live fish or two-photon laser, which allows the reconstruction of three-dimensional pictures of nerve cells. Then, I was awarded with a Larvanet project in the Aquaculture Laboratory of the University of Rome (Italy). The objective of this postdoctoral position was to study the effect of phospholipids and vitamin E on early bone formation and skeleton anomalies in sea bream larvae, learning techniques for the skeletal anomalies evaluation. My main research interest is to understand the connection between essential fatty acids and neural stem cells proliferation and regeneration.
SALEH, R., BETANCOR, M.B., ROO, J., BENITEZ-SANTANA, T., HERNÁNDEZ-CRUZ, CM., MOYANO, J. and IZQUIERDO, M.S. 2013. Optimum krill phospholipids contents in microdiets for gilthead sea bream (Sparus aurata) larvae. Aquaculture Nutrition. doi: 10.1111/j.1365-2095.2012.00976.x.
BENITEZ-SANTANA, T., JUÁREZ-CARRILLO, E., BETANCOR, MB., TORRECILLAS, S., CABALLERO, M.J. and IZQUIERDO, MS. 2012. Increased Mauthner cell activity and escaping behaviour in seabream fed polyunsaturated fatty acids. British Journal of Nutrition. 107, 295-301.
BETANCOR, M.B., CABALLERO, M.J., TEROVA, G., SALEH, R., ATALAH, E., BENITEZ-SANTANA, T., BELL, GJ. & IZQUIERDO, M.S. 2012. Selenium inclusion decreases oxidative stress indicatiors and muscle injuries in sea bas larvae fed higj-DHA microdiets. British Journal of Nutrition doi:10.1017/S0007114512000311.
ERYALÇIN, KM., ROO, J., SALEH, R., BETANCOR, MB., ATALAH, E., BENITEZ-SANTANA, T., CABALLERO, MJ., HERNÁNDEZ-CRUZ, CM. and IZQUIERDO, MS. 2012. Fish oil replacement by different microalgal products in microdiets for early weaning of giltheal seabream (Sparus aurata). Aquaculture Research DOI: 10.1111/j.1365-2109.2012.03237.x.
BETANCOR, MB., CABALLERO, M.J., BENITEZ-SANTANA, T., SALEH, R., ROO, J., ATALAH, E. and IZQUIERDO, MS. 2012. Oxidative status and histological changes in Sea bass larvae muscle in response to high dietary content of DHA. Journal Fish Diseases doi:10.1111/j.1365-2761.2012.01447.x.
TORRECILLAS, S., MAKOL, A., BENITEZ-SANTANA, T., CABALLERO, M.J., MONTERO, D., SWEETMAN, J. & IZQUIERDO, M.S. 2011. Reduced gut bacterial translocation in (Dicentrarchus labrax) fed mannan oligosaccharides (MOS). Fish and Shellfish Immunilogy. 30, 674-681.
Best oral presentation in the Annual International Symposium: The Physiology of Fish Behaviour. July 2012, 9-13. Norwich. UK.