AgreenSkills session, year: 1st session, 2016
Receiving laboratory: UMR IJPB, Versailles-Grignon
Country of origin : USA
The Plant Plugins: The Assembly of Cell Wall Papillae Polymers and the Underlying Cellular Response during Plant Defense
Plant cell walls are the first line of plant defense against pathogens. The cell wall papillae are complex structures differentiated in response to invading pathogens including fungi and bacteria1. Early and rapid papillae formation is critical for successful plant defense. Several cell wall polymers such as callose, cellulose, xyloglucans and lignins are also commonly found in papillae. However, little is known about the interactions of these polymers and the cellular processes involved in papillae assembly, hampering our understanding of the functions of papillae components and the defense mechanisms activated by the perception of pathogens. Using the model pathosystem consisting of the host Arabidopsis thaliana and the fungal pathogen Colletotrichum, we will study the structure-function relationship of papillae polymers and monitor the critical cellular processes that are mobilized for papillae assembly. We will also compare the differences in papillae structure and cellular processes triggered by non-adapted and adapted pathogens, in order to identify the targets of adapted pathogens to suppress papillae formation. Finally we will assess the role of cell wall signaling in papillae formation and how cell wall-derived signals are perceived during fungal infection. A detailed understanding of the function of cell wall papillae and the molecular mechanisms underlying plant defense will provide new prospects for improving food, fiber and biofuel crop production, and thus lead to develop an environmentally sustainable agriculture.
I am a plant biologist with background in biophysics, in biochemistry, and in cell and developmental biology. During my PhD, I pioneered and led the development of atomic force microscopy (AFM) imaging of plant cell walls in fluid. The application of AFM imaging in never-dried plant cell walls promotes our understanding of the molecular structure of plant cell walls, thus providing the foundation for developing sustainable biofuels or creating bio-inspired materials. My current interests focus on cell wall as the interface between plants and pathogenic fungus. How plants respond to fungal invasion and mobilize cell wall polymers to defend fungal attack are the major questions being addressed.
*Zhang T, Vavylonis D, Durachko DM, Cosgrove DJ. Nanoscale movement of cellulose microfibrils in primary cell walls. (2017) Nature Plants (accepted);
*Zhang T, Zheng YZ, Cosgrove DJ. (2016) Spatial organization of cellulose microfibrils and matrix polysaccharides in primary plant cell walls as imaged by multi-channel atomic force microscopy. Plant J. 85: 179-192. (Featured article);
*Xiao CW, Zhang T, Zheng YZ, Cosgrove DJ, Anderson CT. (2016) Xyloglucan deficiency disrupts microtubule stability and cellulose biosynthesis in Arabidopsis, altering cell growth and morphogenesis. Plant Physiol. 170: 234-249;
*Lei L, Zhang T, Strasser R, Lee CM, Gonneau M, Mach L, Vernhettes S, Kim SH, Cosgrove DJ, Li SD, Gu Y. (2014) The jiaoyao1 mutant is an allele of korrigan1 that abolishes endoglucanase activity and affects the organization of both cellulose microfibrils and microtubules. The Plant Cell. 26: 2601-2616;
*Zhang T, Mahgsoudy-Louyeh S, Tittmann B, Cosgrove DJ. (2014) Visualization of the nanoscale pattern of recently-deposited cellulose microfibrils and matrix materials in never-dried primary walls of the onion epidermis. Cellulose. 21: 853-862;
Guo B, Zhang T, Shi J, Chen D, Shen D, Ming F. (2008) Cloning and characterization of a novel PI-like MADS-box gene in Phalaenopsis orchid. DNA Sequence. 19: 332-339;
Guo B, Hexige S, Zhang T, Pittman JK, Chen D, Ming F. (2007) Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid. J Biochem Mol Biol. 40: 845-852;
Chen D, Guo B, Hexige S, Zhang T, Shen D, Ming F. (2007) SQUA-like genes in the orchid Phalaenopsis are expressed in both vegetative and reproductive tissues. Planta. 226: 369-380.
2016 Featured article and cover of The Plant Journal (vol. 85).
2015 Outstanding poster, Gordon Research Conference on Plant Cell Walls, Watham, MA, USA.
2011 Honorable Mention for ChloroFilms, American Society of Plant Biologists.
2008 University Graduate Fellowship Award, Penn State University, USA.
2007 Chun-Tsung Scholar Honors Program, Fudan University, China
2005 Wang-Dao Presidential Scholar Award, Fudan University, China.