Maria Jose Clemente Moreno
AgreenSkills session, year: 1st session, 2012
Receiving laboratory: EGFV Ecophysiology and Grape Functional Genomics Bordeaux Aquitaine, France
Country of origin : Spain
Symplastic cross-talk at the graft interface of grapevine: probing plasmodesmata with state-of-the-art fluorescence technologies
The objective of my AgreenSkills postdoctoral project was to develop an in vitro callus assay to recreate a ‘callus graft’ interface in tissue culture and in vitro micro-grafting in grapevine. Grafting is widely used in the agriculture of fruit crops but it is poorly understand yet. We proposed to study the role of plasmodesmata connections between the scion and rootstock in grafted plant development. This project was part of a larger project of the receiving laboratory, GRAFTACTIONS, which is concerned with the gene expression, protein accumulation and structural developments at the graft interface in different scion/rootstock combinations of grapevine.
I studied biology from 2001 to 2006 in University of Murcia (Spain). In 2007, I started my PhD studies in Plant Biotechnology in Biotechnology of fruit tree Group (Plant Breeding Department) in the CEBAS-CSIC Research Center, under supervision of Dr. Jose Antonio Hernández Cortés. During my PhD, my work was focused in plant response to biotic stress caused by Plum pox virus infection at antioxidant systems level, especially in woody plants peach. I defend my PhD Thesis entitled “Benzothiadiazole (BTH) and L-2-oxothiazolidine-4-carboxylic acid (OTC) effect in the Plum pox virus (PPV) infection to peach and pea plants at subcellular level. Glutathione and related enzymes functions” at 21nd July of 2011. After, thanks to Agreenskills program, I was working in a very innovative and ambitious project as a post-doctoral researcher in Ecophysiologie et Génomique Fonctionnelle de la Vigne group (UMR 1287 -EGFV), in INRA Bordeaux. This project was focused on the study of plasmodesmata connectivity at the graft interface and on the keys steps in the grafting process. The main object were to determine whether the plasmodesmata connections formed between the scion and rootstock of grafted grapevines differed in different scion/rootstock combinations, and to identify gene expression changes induced by symplastic connections between self and non-self genotypes.
Currently, I’m working in the University of the Balearic Islands as post-doc researcher, focused in the eco-physiological and biochemical implications of drought tolerance in grapevine plants. Moreover, although I’ve changed my study topics, I keep in touch with my colleagues in EGFV in order to continue working to finish our project.
GARCÍA-ALMODÓVAR RC, CLEMENTE-MORENO MJ, DÍAZ-VIVANCOS P, PETRI C, RUBIO M, PADILLA IMG, ILARDI V, BURGOS L. (2014) Genetically engineered h-UTR/P1 plum plants displayed sharka resistance under in vitro and greenhouse conditions. PCTOC 120 (2) 791-796. Doi: 10.1007/s11240-014-0629-7.
CLEMENTE MORENO MJ, HERNANDEZ J.A., DIAZ-VIVANCOS P. (2014) Sharka: How plants respond to Plum pox virus infection? J. Exp. Bot. 66(1):25-35. doi: 10.1093/jxb/eru428
CLEMENTE MORENO MJ, HEVIN C, OLLAT N, COOKSON SJ. (2014) Developments at the graft interface in homo- and hetero-grafts: gene expression and histological changes during the first month after grafting. Plant Signaling & Behavior. 25; 9: doi:10.4161/psb.28852 / e28852 (addendum)
COOKSON SJ, CLEMENTE MORENO MJ, HEVIN C, MENDOME LZN, DELROT S, MAGNIN N, TROSSAT-MAGNIN C, OLLAT N (2014) Hetero-grafting with non-self rootstocks induces genes involved in stress responses at the graft interface when compared with auto-grafted controls. J. Exp. Bot. 65(9): 2473–2481. doi: 10.1093/jxb/eru145
SARAH JANE COOKSON; MARIA JOSÉ CLEMENTE MORENO; CYRIL HEVIN; LARISSA ZITA NYAMBA MENDOME; SERGE DELROT; CLAUDINE TROSSAT-MAGNIN AND NATHALIE OLLAT. (2013). Graft union formation in grapevine induces transcriptional changes related to cell wall modification, wounding, hormone signalling, and secondary metabolism.
- Exp. Bot. 64 (10): 2997–3008 DOI:10.1093/jxb/ert144.
CLEMENTE-MORENO MJ; DIAZ-VIVIANCOS P; RUBIO M; FERNANDEZ-GARCIA N; HERNANDEZ JA. (2013). Chloroplast protection in PPV-infected peach plants by OTC treatments: Effect in the proteome. Plant Cell and Environm 36:640-654. DOI: 10.1111/pce.12003.
CLEMENTE-MORENO MJ; DIAZ-VIVIANCOS P; PIQUERAS A; HERNANDEZ JA. (2012) Plant growth stimulation in Prunus species plantlets by BTH or OTC treatments under in vitro conditions.
- plant physiol. http://dx.doi.org/10.1016/j.jplph.2012.03.012.
CLEMENTE-MORENO, M.J.; PIQUERAS, A.; HERNANDEZ, J.A. (2011) Implication of peroxidase activity in development of healthy and PPV-infected micropropagated GF305 peach plants.
Plant Growth Regulation. 65 (2) : 359-367. DOI: 10.1007/s10725-011-9608-8.
CLEMENTE-MORENO, M.J.; DIAZ-VIVANCOS, P.; BARBA-ESPIN, G.; HERNÁNDEZ, J.A. (2010) Benzothiadiazole and L-2-oxothiazolidine-4-carboxylic acid reduced the severity of Sharka symptoms in pea leaves: effect on the antioxidative metabolism at subcellular level. Plant Biology 12: 88-97. DOI: 10.1111/j.1438-8677.2009.00204.x.
Patent entitled: “Treatments to increase vigour in fruit trees under in vitro conditions” (submitted 2011). Ref: P201130888.