AgreenSkills session, year: 2nd session, 2012
Receiving laboratory: IaM Tree/Microorganism Interactions Nancy, France
Country of origin : USA
Adaptation of poplar rust to the poplar varietal landscape: an integrated approach of population genomics, landscape epidemiology and evolution of life history traits
I joined the Tree-Microbe Interactions group via an AgreenSkills fellowship, to study the evolution of poplar rust (Melampsora larici-populina) in response to human-bred disease resistance in poplar plantations. My project here has two aims: first, we will build on the work of Xhaard et al. (2011, Mol Ecol 20: 2739-2755), which documented a landscape-scale takeover by a single genetic group of rust, virulent to a widely planted (and previously rust-resistant) poplar clone.
We will examine the evolutionary dynamics since the takeover, documenting whether introgression of the virulence genes has been influenced by the presence of other resistance types, and whether these types are exerting their own selective pressure.
Secondly, we will shift our focus to quantitative resistance to rust in poplar, that is, differences in degree of disease. Quantitative resistance is believed to be more durable to pathogen evolution than is qualitative, all-or-nothing, disease resistance. Using historic collections, we will study the interactions of clonal rust lineages (taken from across space and time) with quantitatively resistant poplars.
This research will shed light on the ways in which pathogens evolve in response to quantitative resistance, broadening our understanding of host-pathogen interactions while providing information of immediate agronomic utility.
My research is focused on bridging the gap between evolutionary theory and the management of natural systems. Each field can inform the other – management is improved with a science-based perspective, and management situations provide an opportunity to test models in the real world. My doctoral and postdoctoral research with Dr Matto Garbelotto at the Department of Environmental Science, Policy and Management at the University of California, Berkeley, centred on the interaction of tanoak, a unique California native tree, with the recently emerged disease sudden oak death. This system allowed me to study host-pathogen interactions, invasion biology, and emergent diseases using methods ranging from classical quantitative genetics to next-generation sequencing, genomics, and transcriptomics. Prior to my work at Berkeley, I was introduced to plant ecology and pathology in the labs of Drs Ingrid Parker and Greg Gilbert at UC Santa Cruz, where I received a BA in Molecular, Cellular, and Developmental Biology in 2002.
Hayden, K.J., Hardy, G.E.St.J., Garbelotto, M. Oomycete Diseases. In press. In: Gonthier, P., Nicoletti, G. (Eds.), Infectious Forest Diseases, CABI, Oxfordshire, UK.
Hayden, K.J., Garbelotto, M., Dodd, D., Wright, J.W. 2013. Scaling up from greenhouse to field resistance to an introduced forest disease reveals the potential for evolution and management. Evolutionary Applications. doi:10.1111/eva.12080.
Rai, H., Mock, K., Richardson, B., Cronn, R., Hayden, K., Wright, J., Knaus, B., Wolf, P. 2013 Transcriptome characterization and detection of gene expression differences in aspen (Populus tremuloides). Tree Genetics and Genomes. doi: 10.1007/s11295-013-0615-y.
Cobb, R., Metz, M., Frangioso, K, Garbelotto, M., Hayden, K.J., Rizzo, M. 2013. Biodiversity conservation in the face of dramatic forest disease: an integrated conservation strategy for tanoak (Notholithocarpus densiflorus) threatened by sudden oak death. Madroño 60: 151-164.
Garbelotto, M. and Hayden, K.J. 2012. Sudden Oak Death: interactions of the exotic oomycete Phytophthora ramorum with naïve North American hosts. Eukaryotic Cell doi: 10.1128/EC.00195-12.
Osmundson, T., Eyre, C., Hayden, K., Dhillon, J., Garbelotto, M. 2013 Back to basics: An evaluation of NaOH and alternative rapid DNA extraction protocols for DNA barcoding, genotyping, and disease diagnostics from fungal and oomycete samples. Molecular Ecology Resources.
Hayden, K.J., Nettel, A., Dodd, R., Garbelotto, M. 2011. Will all the trees fall? Variable resistance to an introduced forest disease in a highly susceptible host. Forest Ecology and Management 261: 1781-1791.
Hüberli, D., Hayden, K.J., Calver, M., Garbelotto, M. 2011. Intraspecific variation in host susceptibility and climatic factors mediate the progression of sudden oak death in US woodlands. Plant Pathology doi: 10.1111/j.1365-3059.2011.02535.x.
Hayden, K.J., Ivors, K., Wilkinson, C., Garbelotto, M. 2006. TaqMan chemistry for Phytophthora ramorum detection and quantification, with a comparison of diagnostic methods. Phytopathology 96: 846-854.
Swain, S., Harnik, T., Mejia-Chang, M., Hayden, K., Bakx, W., Creque, J., Garbelotto, M. 2006. Composting is an effective treatment option for sanitization of Phytophthora ramorum-infected plant material. Journal of Applied Microbiology 101: 815-827.
Hayden, K.J., Rizzo, D., Tse, J., Garbelotto, M. 2004. Detection and quantification of Phytophthora ramorum from California forests using a real-time polymerase chain reaction assay. Phytopathology 94: 1075-1083.
Ivors, K.L., Hayden, K.J., Bonants, P.J.M., Rizzo, D.M., Garbelotto, M. 2004. AFLP and phylogenetic analyses of North America and European populations of Phytophthora ramorum. Plant Disease 89: 204.
Hayden, K.J., Parker, I.M. 2002. Plasticity in cyanogenesis of Trifolium repens L: Inducibility, fitness costs, and variable expression. Evolutionary Ecological Research 4: 155-168.