Karine Prado

Karine Prado

AgreenSkills session, year: 2nd session, 2013

Receiving laboratory: SynthSys, Edinburgh, United Kingdom

Country of origin : France




Mobility project

Study of the contribution of non-transcriptional mechanisms to biological timekeeping from Ostreococcus tauri to Arabidopsis thaliana

The clock mechanisms have been described as a complex network of multiple interconnected transcriptional/translational feedback loops controlling a wide variety of vital processes on organism, tissue, cellular and molecular levels. New evidences indicate a non-transcriptional timing, well known in prokaryotes, is also present in eukaryotes. The aims are to characterize for the first time in eukaryotes the ancestral non-transcriptional clock and to determine how protein phosphorylation affects the clock system. The mechanisms will be described firstly using a reduced model system, the pico-alga Ostreococcus tauri, and then confirmed in model plant Arabidopsis thaliana.

Biography & research interests

I studied Biology Science at the University of Montpellier 2 where I obtained an MRes in Plant Functional Biology in 2010, then a doctoral fellowship from INRA (“Contrat Jeune Scientifique”). My PhD was carried out at INRA of Montpellier in the lab “Biochimie et Physiologie Moléculaire des plantes” (B&PMP) under the supervision of Dr Christophe Maurel and was aimed to contribute to determine the genetic, molecular and cellular bases of water transport regulation by water channel aquaporins in leaves of Arabidopsis thaliana. After my PhD defense at the end of 2013, I pursued my CJS contract in joining Professor Andrew Millar’s lab in SynthSys lab of University of Edinburgh in United Kingdom as a post-doctoral associate.  I am studying the contribution of non-transcriptional mechanisms to biological timekeeping from a pico-alga Ostreococcus tauri to Arabidopsis thaliana. With a small genome, little genetic redundancy and conserved regulators with the plant circadian clock, O. tauri is an ideal model organism for plant systems biology. Through the post-doctoral project, I continue to enrich my knowledge about the post-translational regulation but in a larger, proteome-wide scale than during my PhD project focusing on one specific protein. In the same time, because the circadian clock is a good system to study the Systems Biology, I learn the fundamentals of this recent thematic for which the interest growths along with the increasing of bioinformatics data.

Selected publications

Tsuchihira A, Prado K, Hanba Y, Mori IC, Maurel C, Maeshima M. Extensive and transient gene expression enhancement of the plasma membrane aquaporin PIP2;3 in response to high temperatures in Arabidopsis thaliana. In prep for Plant Cell journal

Ronzier E, Corratge-Faillie C, Sanchez F, Prado K, Brierez C, Leonhardt N, Thibaud JB, Xiong TC (2014) CPK13, a non-canonical CPK, specifically inhibits KAT1 and KAT2 Shaker channels and reduces stomatal opening. Submitted in Plant physiol journal

di Pietro M, Vialaret J, Li G, Hem S, Prado K, Rossignol M, Maurel C, Santoni V (2013) Coordinated post-translational responses of aquaporins to abiotic and nutritional stimuli in Arabidopsis roots. Mol Cell Proteomics. 12:3886-97

Prado K, Maurel C (2013) Regulation of leaf hydraulics: from molecular to whole plant levels. Front. Plant Sci. 4: 255

Prado K, Boursiac Y, Tournaire-Roux C, Monneuse JM, Postaire O, Da Ines O, Schaffner AR, Hem S, Santoni V, Maurel C (2013) Regulation of Arabidopsis leaf hydraulics involves light-dependent phosphorylation of aquaporins in veins. Plant Cell 25: 1029-39



Website(s): http://www.synthsys.ed.ac.uk/