AgreenSkills session, year: 2nd session, 2015
Receiving laboratory: CBGP Centre for Biology and Management of Populations, Montpellier
Country of origin : Australia
Unravelling the genetic covariance structure of resistance traits expressed in different life stages of a model pest insect to design sustainable biocontrol strategies
The design of sustainable biocontrol strategies requires taking into account the evolutionary response of pests against biocontrol agents (i.e. predators and parasites). The emergence of pest counter-adaptation critically depends on the costs associated with the defence against these agents. However, costs expressed in life stages that are not attacked by focal biocontrol agents have been largely overlooked. The genetic architecture of resistance traits expressed at different stages needs investigating to reveal whether shared genetic control leads to coupled evolution among traits separated by metamorphosis. The aim of the project is to survey the extent of genetic coupling across metamorphosis of many traits, in particular those involved in immune defence. We will use state-of-the-art quantitative genetics applied to transcriptomics data and artificially selected lines to investigate the extent of genetic co-variance across metamorphosis, using a model species Drosophila melanogaster.
First, I am studying the transcriptome of larvae and adults of in bred lines of D. melanogaster within the G-matrix framework to reveal the evolutionary coupling between life stages in numerous traits. Focusing on resistance traits, I can evaluate the costs of resistance in response to different biocontrol agents targeting specific stages of a pest. Second, I am using artificially selected lines that are resistant to a parasitoid. By studying whether adult resistance traits are affected by the selection regime, I will reveal the genetic constraints of defence functions between life stages, to help identify the most evolutionary sustainable bioagent to fight against pests with several life stages.
I am an evolutionary biologist interested in genetic constrains on phenotype evolution. I am particularly interested in how pleiotropy between traits separated by life-stages and traits alternatively expressed in males and females limit the response to the different selection forces experienced during life and between sexes.
I am currently a Marie Curie fellow at the CEFE (CNRS, Montpellier) and my project focusses on understanding sexual selection to help controlling an invasive pest species, Drosophila suzukii. I previously was an Agreenskills plus fellow investigating the genetic covariances of traits in different life stages (INRA, France), using the D. melanogaster as a model. Before that, I combined quantitative genetic methods with gene expression patterns in D. serrata to understand the effect of sexual selection on mutational pleiotropy at the University of Queensland (Australia). During my first post-doctoral position (University College London, UK), I used quantitative genetics and molecular methods to investigate the rapid evolution of intra-locus sexual conflict in D. melanogaster. Previously, my PhD research focused on the sexual selection acting on red junglefowl (Gallus gallus) phenotypes (University of Oxford, UK).
Collet J. M., Fuentes S., Hesketh J., Hill M. S., Innocenti P., Morrow E.H., Fowler K., and M. Reuter (2016) “Rapid evolution of the inter-sexual genetic correlation for fitness in Drosophila melanogaster.” Evolution 70 (4): 781-795.
* Collet J. M., Blows M. W., and K. McGuigan.(2015) “Transcriptome-wide effects of sexual selection on the fate of new mutations.” Evolution 69 (11):2905-2916.
Blows M. W., Allen S. L., Collet J. M., Chenoweth S. F., and K. McGuigan. (2015) “The phenome-wide distribution of genetic variance.” Am Nat 186 (1): 15-30.
Collet J. M. and M. W. Blows. (2014) “Female mate choice predicts paternity success in the absence of additive genetic variance for other female paternity bias mechanisms in Drosophila serrata.” J Evol Biol 27 (11): 2568-2572.
* McGuigan K., Collet J. M., Allen S. L., Chenoweth S. F., and M. W. Blows. (2014) “Pleiotropic mutations are subject to strong stabilising selection” Genetics 197 (3): 1051-1062.
* Collet J. M., Dean R. F., Worley K., Richardson D. S., and T. Pizzari. (2014) “The measure and significance of Bateman’s principles.” Proc Roy Soc B: Biol Sci 281 (1782).
* McGuigan K., Collet J. M., McGraw E. A., Ye Y. H., Allen S. L., Chenoweth S. F., and M. W. Blows (2014) “The nature and extent of mutational pleiotropy in gene expression of male Drosophila serrata.” Genetics 196 (3): 911-921.
* Collet J., Richardson D., Worley K., and T. Pizzari. (2012) “Sexual selection and the differential effect of polyandry.” Proc Natl Acad Sci USA 109 (22): 8641-8645.
Worley K., Collet J., Spurgin L. G., Cornwallis C., Pizzari T., and D. S. Richardson. (2010) “MHC heterozygosity and survival in red jungle fowl.” Mol Ecol 19(15): 3064-3075.
2018/2020 Marie Skłodowska-Curie Individual-Fellowship. Reintegration panel. €173,076. Rated 96/100;
2016/2018 Agreenskills fellowship. Ranked second best Incoming project. AgreenSkills is an international postdoctoral fellowship programme co-funded by the European Union and coordinated by INRA , in collaboration with Agreenium-IAVFF , the French national higher education and research consortium. The programme promotes international mobility for experienced young and independent researchers to develop challenging basic or targeted research projects in the fields of agriculture, food, nutrition, environment, and animal health;
2006/2009 BBSRC Studentship. I received a fees only award (in open competition) due to non-UK residency. Independently I obtained all the funds necessary for my stipend for the duration of my studies;
2007/2008 Light Senior Scholarship from St. Catherine’s College. Small grant (£350) awarded to the best candidates across disciplines, open to all Graduate students of the University of Oxford;
2006 ASAB Conference Grant: Award to help postgraduate students, and others, attend ASAB conferences.