AgreenSkills session, year: 1st session, 2016
Receiving laboratory: UNH Human nutrition Clermont-Ferrand-Theix
Country of origin : Germany
Development of databases on food metabolome
The quantification of food intake is an important part of nutrition studies, normally done using food frequency questionaires. Unfortunately, this method is prone to under or overreporting of food intake, compromising the interpretation of results in many cases. Markers of food intake are the best alternative to overcome this problem as they provide a direct measure of food consumption, allowing more accuracy in nutrition cohorts. So far, only few food items are covered by validated biomarkers of intake and the discovery of novel markers is necessary to push nutrition research forward.
The present proposal is part of a EU-funded project – FoodBall (Food Biomarkers Alliance) aiming to develop the missing tools and resources to facilitate the identification of biomarkers of food intake using metabolomics. It includes the main tasks described bellow:
Construction of a database on food metabolome:
The database will contain chemical and biochemical data on all known dietary compounds, the foods where they are found as well as their concentration. It will include both known (reported in the literature) and in silico predicted human metabolites of dietary compounds.
Development of a library for food-derived metabolites
A new chemical library will be created including standards for food compounds and their metabolites as well as directories of laboratories where these standards may be obtained. Food compound metabolites will also be directly obtained through in vivo biotransformation of specific food constituents. Reference MS and NMR spectral information will be collected and made available.
The tools described above will be available to the scientific community through a public web portal which will also include any further information considered useful or that may facilitate the identification and use of dietary biomarkers and the interaction of the different groups.
I studied Biological Sciences at the Universidade do Vale do Itajaí (Itajaí, Brazil) and received the PhD degree from the department of Physioloy and Biophysics at the University of São Paulo (São Paulo, Brazil). The topic of my thesis was the study of n-3PUFA effects on energy metabolism and type-2 diabetes in mice. After the PhD, I started a post-doc at the University of São Paulo, investigating the relationship between n-3PUFA and energy metabolism, but focusing on hepatic fatty acid oxidation. In this period, I joined the chair of Nutritional Physiology at the Technical University of Munich (Fresing, Germany), as a visiting scientist, applying mass spectrometry tools to investigate intermediate metabolism in mice. In January 2014, I was hired as a post-doc at the Technical University of Munich to use different mass spectrometry-based platforms in the study of post-prandial metabolism, as part of the Nutritech project. In June 2016, I joined the team of Claudine Manach at INRA (Saint-Genés Champanelle, France), being involved in the activities of the FoodBAll project. I am currently using in silico predictions of metabolism to help the identification of new metabolites from terpenes and I am collaborating in the development of a chemical library (FoodComEx).
* Fiamoncini J, Turner N, Hirabara SM, Salgado TML, Marçal AC, Leslie S et al. Enhanced peroxisomal β-oxidation is associated with prevention of obesity and glucose intolerance by fish oil-enriched diets. Obesity 2013; 21: 1200–1207. doi:10.1002/oby.20132
* Fiamoncini J, Lima TM, Hirabara SM, Ecker J, Gorjão R, Romanatto T et al. Medium-chain dicarboxylic acylcarnitines as markers of n-3PUFA-induced peroxisomal oxidation of fatty acids. Mol Nutr Food Res 2015; 00: 1-11. doi:10.1002/mnfr.201400743.
* Müller VM, Zietek T, Rohm F, Fiamoncini J, Lagkouvardos I, Haller D et al. Gut barrier impairment by high-fat diet in mice depends on housing conditions. Mol Nutr Food Res 2016; 60: 897–908. doi:10.1002/mnfr.201500775
* Romanatto T, Fiamoncini J, Wang B, Curi R, Kang JX. Elevated tissue omega-3 fatty acid status prevents age-related glucose intolerance in fat-1 transgenic mice. Biochim Biophys Acta 2014; 1842: 186-191. doi:10.1016/j.bbadis.2013.10.017.
* Hirabara SM, Folador A, Fiamoncini J, Lambertucci RH, Rodrigues CF, Rocha MS et al. Fish oil supplementation for two generations increases insulin sensitivity in rats. J Nutr Biochem 2013; 24: 1136–1145. doi:10.1016/j.jnutbio.2012.08.014