AgreenSkills session, year: 2nd session, 2015
Receiving laboratory: Department of Plant Biology, Carnegie Institution for Science, USA
Country of origin : France
Systems biology approaches to identify regulatory networks that integrate the response of plants to multiple nutritional stresses: a case study in phosphorus, zinc, and iron
Phosphate (Pi) is an essential macronutrient for plant growth. Plants acquire Pi through roots, and its deficiency (-Pi) causes a severe reduction in plant development and in particular in primary root growth (PRG). This phenotype appears also to be a result of iron (Fe) excess. But simultaneous Pi and Fe deficiencies (-Pi-Fe) restore the PRG. The role of zinc (Zn) in regulating PRG under -Pi is also emerging, where simultaneous Pi deficiency and excess Zn (-Pi++Zn) restore the PRG, mimicking -Pi-Fe condition. With the established antagonistic interaction between Fe and Zn, these data all point to the existence of a tripartite Pi/Zn/Fe signaling network that crosstalks and regulates PRG under -Pi. Nevertheless, despite its fundamental importance, the molecular mechanisms leading to the integration of these three signals to maintain root growth remains unknown. Therefore, this original “PiZnFe-Net” proposal aims at identifying key regulatory genes and pathways involved in the integration of Pi/Zn/Fe signals in Arabidopsis by employing transcriptomics, computational biology, and reverse genetic approaches. Integration of theses data will provide a blueprint for discovering new regulatory pathways of Pi nutrition in plants.
I gained scientific and managerial skills during my PhD (University of Montpellier II, France, 2002-2005), my Postdoc (University of Lausanne, Switzerland, 2005-2009), and as Senior Scientist in Crop Design (BASF Company, Ghent, Belgium, 2010-2012). In 2010, I obtained the academic diploma of habiliation to direct research work « HDR » (University of Bourgogne, France). During my PhD and postdoctoral periods, I acquired expertise in the area of molecular and physiological aspects of plant nutrition. In particular, I studied the molecular mechanisms controlling the sulfate and phosphate transport and signaling in Arabidopsis through the study of the SULTR and PHO1 gene family. In 2012, I was hired at INRA- Montpellier -FRANCE. Since then, I am developing an original research program in the “Biochemistry and Molecular Biology of Plants” (B&PMP) Research Unit, aiming at decoding the genetic and molecular basis of the interaction between the homeostasis of macro-and micronutrients, particularly phosphate and metals, in plants. In 2016, I was awarded as “Chercheur d’Avenir, Region Longuedoc Roussillon –France). In 2016, I was appointed as Adjunct Professor in Chiang Mai University, Thailand. The same year, I joined Carnegie Institution for Science, Stanford, as AgreenSkills Plus fellow.
Secco D, Whelan J, Rouached H, Lister R. Nutrient stress-induced chromatin changes in plants. Curr Opin Plant Biol. 2017 – 22;39:1-7.
Mongon J, Chaiwong N, Bouain N, Prom-U-Thai C, Secco D, Rouached H. Phosphorus and Iron Deficiencies Influences Rice Shoot Growth in an Oxygen Dependent Manner: Insight from Upland and Lowland Rice. Int J Mol Sci. 2017 – 10;18(3).
Secco D, Bouain N, Rouached A, Prom-U-Thai C, Hanin M, Pandey AK, Rouached H. Phosphate, phytate and phytases in plants: from fundamental knowledge gained in Arabidopsis to potential biotechnological applications in wheat. Crit Rev Biotechnol. 2017 – 12:1-13.
Heuer S, Gaxiola R, Schilling R, Herrera-Estrella L, López-Arredondo D, Wissuwa M, Delhaize E, Rouached H. Improving phosphorus use efficiency: a complex trait with emerging opportunities. Plant J. 2017 in press
Saenchai C, Bouain N, Kisko M, Prom-U-Thai C, Doumas P, Rouached H. The Involvement of OsPHO1;1 in the Regulation of Iron Transport Through Integration of Phosphate and Zinc Deficiency Signaling. Front Plant Sci. 2016 6;7:396.
Belgaroui N, Berthomieu P, Rouached H, Hanin M. The secretion of the bacterial phytase PHY-US417 by Arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co-growth. Plant Biotechnol J. 2016 – 14(9):1914-24. doi: 10.1111/pbi.12552. Epub 2016 Mar 30.
Briat JF, Rouached H, Tissot N, Gaymard F, Dubos C. Integration of P, S, Fe, and Zn nutrition signals in Arabidopsis thaliana: potential involvement of PHOSPHATE STARVATION RESPONSE 1 (PHR1). Front Plant Sci. 2015 – 28;6:290.
Belgaroui N, Zaidi I, Farhat A, Chouayekh H, Bouain N, Chay S, Curie C, Mari S, Masmoudi K, Davidian JC, Berthomieu P, Rouached H, Hanin M. Over-expression of the bacterial phytase US417 in Arabidopsis reduces the concentration of phytic acid and reveals its involvement in the regulation of sulfate and phosphate homeostasis and signaling. Plant Cell Physiol. 2014 -55(11):1912-24. doi: 10.1093/pcp/pcu122. Epub 2014 Sep 16.
Khan GA, Bouraine S, Wege S, Li Y, de Carbonnel M, Berthomieu P, Poirier Y, Rouached H. Coordination between zinc and phosphate homeostasis involves the transcription factor PHR1, the phosphate exporter PHO1, and its homologue PHO1;H3 in Arabidopsis. J Exp Bot. 2014 – 65(3):871-84.
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis. Plant J. 2011 – 65(4):557-70.
Rouached H, Secco D, Arpat B, Poirier Y. The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis. BMC Plant Biol. 2011 – 24;11:19.
2001-2005: Excellence fellowship for 4 years (Ministry of higher education and scientific research in Tunisia)
2011: Selected Scientist among CropDesign employees for the BASF Plant Science University.
2016: Promising Researchers at the region of Languedoc- Roussillon (Chercheur d’avenir de la Région Languedoc-Roussillon edition of 2016).