AgreenSkills session, year: 1st session, 2017
Receiving laboratory: GABI and VIM units, INRA Jouy-en-Josas
Country of origin : Sweden
P.I.G. Pig Immunity Genomics
Viruses can trigger the down-regulation of host immune responses by several immune evasion strategies. Dendritic cells (DCs) and macrophages (Mɸ) are fundamental components of the innate immunity and are among the first cells interacting with pathogens in mucosae. Together they form a cellular network involved in pathogen sensing, controlling inflammation and initiating the adaptive immune responses. The Porcine Reproductive and Respiratory Syndrome virus (PRRSV) participates in a variety of polymicrobial syndromes and is an economically significant disease impacting pig production worldwide. The recent emergence of highly pathogenic PRRSV strains underlines the need for a comprehensive understanding of the anti-PRRSV host immune responses to enable the design of effective vaccines. In this context, INRA is leading the SAPHIR EU project, involved in the concept of new PRRSV vaccines, and is partner of the ANIHWA project ‘KILLeuPRRSV’, whose main aim is to unravel the mechanisms of interaction underlying PRRSV’s immune-evasion strategy. P.I.G. project will finely dissect the mechanisms of interaction triggered by PRRSV by combining immunological (VIM Unit, SA Div.) and functional genomics (GABI Unit, GA Div.) approaches. Alterations of Mɸ/DC subpopulations from the lung upon infection with virulent PRRSV strains will be characterized by phenotype and function (cytokine production and antigen presentation capacity), coupled to RNAseq to identify common/specific transcriptional signatures and regulatory pathways altered by PRRSV. Working with this refined experimental system will allow me to enrich my professional expertise in animal viro-immunology and it will open to new projects of disease functional genomics in an applied animal science context.
I think about myself as a “viro-immunologist” working mainly in animal science. I’m a veterinarian with expertise in host-pathogen interaction, in particular in swine virology and immunology.
I got my DVM at the University of Bologna (2005, Italy) and I did my MSc and my PhD in Veterinary Health and Medicine till 2011 at CRESA (Animal Research Centre in Barcelona, Spain).
I started to be interested in virology and pigs during my final degree project in 2004 when I was studying porcine circovirosis. Then I continued in pig viro-immunology during my PhD thesis, focused mainly on virus like particles studies as vaccine vector for animal viral diseases. I worked with different porcine viruses: Influenza virus, circovirus, PRRSV, FMDV and CSF and also under high biosafety conditions with Biolevel 3 pathogens. I was collaborating in applied virology with stakeholders and different companies, to develop the safety study for a viral vaccine (Pfizer), to set up experimental viral infection (HIPRA) or to test immunomodulatory compound (Maymo) to improve porcine industry.
From 2013 till 2016 I started to work in human virology at Linköping University (Sweden) where I was studying the interaction between human viruses (HSV2, HIV), immune cells and complement system using next generation sequencing.
Currently I’m working at INRA Jouy-en-Josas developing the P.I.G. project that covers the last period of the KILLeuPRRSV project, which spanned a total of 4 years. P.I.G. will contribute to complete the knowledge on the immunological alterations of the different PRRSV strains on the respiratory immune system.
1. Yap SH, Abdullah NK, McStea M, Takayama K, Chong ML, Crisci E, Larsson M, Azwa I, Kamarulzaman A, Leong KH, Woo YL, Rajasuriar R . HIV/Human herpesvirus co-infections: impact on tryptophan-kynurenine pathway and immune reconstitution. PLoS One. 2017 Oct 9;12(10):e0186000. doi: 10.1371/journal.pone.0186000.
2. N. Moreno, I. Mena, I. Angulo, Y. Gómez, E. Crisci, M. Montoya, J. Caston, E. Blanco and J. Barcena. Rabbit hemorrhagic disease virus capsid, a versatile platform for foreign B-cell epitope display inducing protective humoral immune responses. Scientific Reports 2016 Aug 23; 6:31844. doi: 10-1038/srep31844
3*. E. Crisci, R. Ellegård, S. Nyström, E. Rondahl, L. Serrander, T. Bergström, C. Sjöwall, K. Eriksson and M. Larsson. Complement opsonization promotes HSV-2 infection of human dendritic cells. Journal of Virology 2016 Apr 29;90(10):4939-50. doi: 10.1128/JVI.00224-16. Print 2016 May 15.
4*. Maisonnasse P, Bouguyon E, Piton G, Ezquerra A, Urien C, Bourge M, Deloizy C.,Leplat JJ, Simon G, Chevalier C, Vincent-Naulleau S, Crisci E, Montoya M, Schwartz-Cornil I, Bertho N. The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model. Mucosal Immunol. 2016 Jul;9(4):835-49. doi: 10.1038/mi.2015.105. Epub 2015 Nov 4.
5. Ellegård R., Crisci E, Andersson J, Shankar EM, Hinkula J, Lifson JD, Larsson M. Impaired natural killer cell activation and chemotaxis towards dendritic cells exposed to complement opsonized HIV-1.J Immunology 2015 Aug 15, 195(4): 1698-704.
6*. E. Crisci, L. Fraile, R. Novellas, Y. Espada, R. Cabezón, J. Martínez, J. Bárcena, D. Benitez-Ribas and M. Montoya.In vivo tracking and immunological properties of pulsed pig-derived dendritic cells. Mol Immunol. 2015, Feb 63(2):343-54.
7. Ellegård R., Crisci E, Burgener A, Hinkula J, Sjövall C, Lifson J, and Larsson M.
Complement Opsonization of HIV-1 Results in Decreased Antiviral and Inflammatory Responses in Immature Dendritic Cells via CR3. J Immunology 2014 Nov 1;193(9):4590-601.
8. E. Crisci, L. Fraile, S. Valentino, L. Martínez, B. Bottazzi, A. Mantovani , M. Montoya.Immune characterization of long pentraxin 3 in pigs infected with influenza virus. Vet Microbiol. 2014 Jan 10;168(1):185-92.
9*. E. Crisci, L. Fraile, N. Moreno, E. Blanco, R. Cabezón, C. Costa, T. Mussá, M. Baratelli, P. Martinez Orellana, J. Martínez, J. Bárcena, M. Montoya.Chimeric calicivirus-like particles elicit specific immune responses in pigs.Vaccine 2012, 30:2427-2439.
10. N. Busquets, J. Segalés, L. Córdoba, T. Mussá, E. Crisci , G.E. Martín-Valls, M. Simon-Grifé, M. Pérez-Simó, M. Pérez-Maíllo, J. I. Núñez, F.X.Abad, L. Fraile, S. Pina, N. Majó, A. Bensaid, M. Domingo, M.Montoya. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet Research 2010 Sep-Oct; 41(5):74.
11. E. Crisci, M. Ballester, J. Domínguez, J. Segalés, M. Montoya. Increased numbers of myeloid and lymphoid IL-10 producing cells in spleen of pigs with naturally occurring postweaning multisystemic wasting syndrome.Vet Immunol Immunopathol. 2010 Aug 15; 136(3-4):305-10.
12. J. Tarradas, J. Marqués, R. Rosell, M. Nofrarías, E. Crisci, L. Córdoba, E. Pérez, I. Díaz, F. Rodríguez, M. Domingo, M. Montoya, L. Ganges. Interferon gamma induction correlates with protection by DNA vaccine expressing E2 glycoprotein against classical swine fever virus infection in domestic pigs. Veterinary Microbiology 2010 Apr 21; 142(1-2):51-8.
13. J.Segales, A. Urniza, A Alegre, T. Bru, E. Crisci, M. Nofrarias, S. Lopez-Soria, Monica Balasch, M.Sibila, Z. Xu, H.J. Chu, L.Fraile, J. Plana-Duran. A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) improves clinical, pathological and virological outcomes in postweaning multisistemic wasting syndrome affected farms. Vaccine 2009; Dec 9; 27(52):7313-21.
14. E. Crisci, H. Almanza, I. Mena, L. Córdoba, E. Gómez-Casado, J.R. Castón, L. Fraile, J. Bárcena, M. Montoya.
Chimeric calicivirus-like particles elicit protective anti-viral cytotoxic responses without adjuvant.
Virology 2009; 387:303-312.
15. L. Grau-Roma, E. Crisci, M. Sibila, S. López-Soria, M. Nofrarias, M. Cortey, L. Fraile, A. Olvera and J. Segalés. A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence.
Vet Microbiol. 2008 Apr 1; 128(1-2):23-35.
1. E. Crisci, T. Mussà, L. Fraile, M. Montoya. Review: Influenza virus in pigs. Review. Molecular Immunology 2013, 55: 200-211.
2*. E. Crisci, J. Bárcena and M. Montoya.Virus-like particles: The new frontier of vaccines for animal viral infections.Review. Vet Immunol Immunopathol. 2012 Aug 15;148(3-4):211-25. Epub 2012 Jun 1. It was first in the most 20 downloaded papers of SciVerse ScienceDirect.
2016. Special Award for the PhD granted by the Universitat Autonoma de Barcelona (UAB) the 8th of April 2016 at Hotel Campus UAB.
2015. Travel award from MiMS (Molecular Infection Medicine Sweden) National Doctoral Programme in Infections and Antibiotics for the 32nd Annual Meeting in NSCMID- Infections and Antibiotics, September 2-6, 2015, Umeå, Sweden.
2013. Travel award from European Federation of Immunological Societies (EFIS) for the 15th International Congress of Immunology (ICI), August 22-27, 2013, Milan, Italy.
2010. Travel award from European Federation of Immunological Societies (EFIS) for the
14th International Congress of Immunology (ICI), August 22-27, 2010, Kobe, Japan.
2009. Travel award from European Federation of Immunological Societies (EFIS) for the 2nd
European Congress of Immunology (ECI), September 13-16, 2009, Berlin, Germany.
Website(s): https://www6.jouy.inra.fr/gabi_eng/ | https://www6.jouy.inra.fr/vim_eng/ | ORCID:000-0002-4235-8508; https://www.researchgate.net/profile/Elisa_Crisci | https://www.linkedin.com/in/elisa-crisci-83181a20/