AgreenSkills session, year: 1st session, 2015
Receiving laboratory: DynAMic Dynamique des Génomes et Adaptation Microbienne, Nancy
Country of origin : Spain
Search for new secondary metabolites biosynthetic genes clusters through the identification and study of genomic islands
Natural products are small chemical compounds produced by living organisms that can have diverse biological activities. Streptomyces, a filamentous soil-dwelling bacteria, is well known for its ability to produce a large variety of secondary metabolites, and genome sequences have shown that a single Streptomyces strain is able to synthesize 20-30 different molecules.
This means a lot more natural products wait to be discovered and yet, estimates show that approximately 90 % of these are “cryptic”, i.e. not produced under standard laboratory conditions. Different approaches have been developed to identify these silent biosynthetic gene clusters, most of which are similarity-based.
This mobility project aims at developing a novel secondary metabolite discovery method based on comparative genomics, an approach that does not require any previous knowledge of the nature of the secondary metabolite gene cluster.
I’m a microbiologist working on molecular microbiology and bioinformatics. My main research interest is the biosynthesis of secondary metabolites in Streptomyces and other actinomycetes, as a means to help in the discovery of new natural products that can have antimicrobial properties. I did my PhD in the University of León (Spain) studying the regulation of polyene macrolide biosynthesis and presently I’m a postdoctoral research fellow at the DynAMIc research unit INRA-University of Lorraine in Nancy (France), studying the production of natural products in Streptomyces ambofaciens.
Aparicio JF, Barreales EG, Payero TD, Vicente CM, de Pedro A, Santos-Aberturas J. (2016). Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation. Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-015-7077-0;
*Payero TD, Vicente CM, Rumbero Á, Barreales EG, Santos-Aberturas J, de Pedro A, Aparicio JF. (2015). Functional analysis of filipin tailoring genes from Streptomyces filipinensis reveals alternative routes in filipin III biosynthesis and yields bioactive derivatives. Microb Cell Fact. doi: 10.1186/s12934-015-0307-4;
*Vicente CM, Payero TD, Santos-Aberturas J, Barreales EG, de Pedro A, Aparicio JF. (2015). Pathway-specific regulation revisited: cross-regulation of multiple disparate gene clusters by PAS-LuxR transcriptional regulators. Appl Microbiol Biotechnol. doi: 10.1007/s00253-015-6472-x;
*Vicente CM, Santos-Aberturas J, Payero TD, Barreales EG, de Pedro A, Aparicio JF. (2014). PAS-LuxR transcriptional control of filipin biosynthesis in S. avermitilis. Appl Microbiol Biotechnol. doi: 10.1007/s00253-014-5998-7;
Santos-Aberturas J, Vicente CM, Payero TD, Martín-Sánchez L, Cañibano C, Martín JF, Aparicio JF. (2012). Hierarchical control on polyene macrolide biosynthesis: PimR modulates pimaricin production via the PAS-LuxR transcriptional activator PimM. PLoS One. 7(6):e38536. doi: 10.1371/journal.pone.0038536;
Guerra SM, Rodríguez-García A, Santos-Aberturas J, Vicente CM, Payero TD, Martín JF, Aparicio JF (2012). LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor. PLoS One. 7(2):e31475. doi: 10.1371/journal.pone.0031475;
*Santos-Aberturas J, Payero TD, Vicente CM, Guerra SM, Cañibano C, Martín JF, Aparicio JF (2011). Functional conservation of PAS-LuxR transcriptional regulators in polyene macrolide biosynthesis. Metab Eng. 13(6):756-67. doi: 10.1016/j.ymben.2011.09.011;
Santos-Aberturas J, Vicente CM, Guerra SM, Payero TD, Martín JF, Aparicio JF (2011). Molecular control of polyene macrolide biosynthesis: direct binding of the regulator PimM to eight promoters of pimaricin genes and identification of binding boxes. J Biol Chem. 18;286(11):9150-61. doi: 10.1074/jbc.M110.182428;
*Vicente CM, Santos-Aberturas J, Guerra SM, Payero TD, Martín JF, Aparicio JF (2009). PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensis. Microb Cell Fact. 8;8:33. doi: 10.1186/1475-2859-8-33.