AgreenSkills session, year: 2nd session, 2013
Receiving laboratory: RDP Plant Reproduction and Development Clermont-Ferrand-Theix, France
Country of origin : Spain
Cell separation as a mechanism underlying tissue ablation in developing seeds
The endosperm is a nourishing tissue present in the developing seed of flowering plants and originating from a product of double fertilization. Unlike in cereals where endosperm represents the major volume of the mature seeds, in Arabidopsis, endosperm is progressively consumed as the embryo develops. This proposal aims to characterize the mechanisms controlling endosperm breakdown through a multidisciplinary approach combining genetic, biochemical, cytological, and biophysical analyses. This project will provide a better understanding of the endosperm breakdown mechanisms in early seed development and could contribute to the long term deployment of novel tools for engineering seed characteristics in crop species.
I am particularly interested in the mechanisms of regulation of plant developmental processes and their evolution. For that reason I chose for my PhD an “evo-devo” thematic focused on carpel development and the origin of the flower (RDP, ENS-Lyon, France). As I am also interested on the applied aspects of research and the potential applications of my work, after my PhD I carried out a short post-doc on a biotechnological research project in partnership with an industry (University of Queensland, Australia). During my second post-doc I combined the different aspects of my previous experiences: flower morphogenesis, “evo-devo” and biotechnology (IBMCP, Spain). I studied fruit morphological diversity and evolution of the carpel and fruit genetic regulation pathways. An important part of my work was performed in Legumes and presented major agronomic applications. Taken together, this research experience has provided me with an extensive training in plant development, functional genetics, molecular regulatory pathways and comparative studies, as well as a very good scientific track (see publications and patents below). I have worked with many plant species (model and non-model) and developed thorough skills in molecular biology, genetics, phenotypic analyses, histology, microscopy, sequences analyses and phylogeny. The AgreenSkills program represents a perfect opportunity to further strengthen my skills and knowledge and put my assets into a very exciting research project in an excellent European research centre.
Fourquin C. and Ferrandiz C. (2014) The essential role of NGATHA genes in style and stigma specification is widely conserved across eudicots. New Phytologist. Jan 31. doi: 10.1111/nph.12703. [Epub ahead of print]
Ferrandiz C. and Fourquin C. (2014) The role of the FUL-SHP network in the evolution of fruit morphology and function. J Exp Bot. Jan 30. [Epub ahead of print]
Balanza V., Ballester P., Colombo M., Fourquin, C., Martínez-Fernández I. and Ferrándiz C. (2014) Genetic and phenotypic analyses of carpel development in Arabidopsis. Methods Mol Biol. 1110, 231-49.
Fourquin C., del Cerro C., Victoria F.C., Vialette-Giraud A., de Oliveira A.C. and Ferrandiz C. (2013) A change in SHATTERPROOF protein lies at the origin of a fruit morphological novelty and a new strategy for seed dispersal in Medicago genus. Plant Physiol 162, 907-917.
Fourquin C. and Ferrandiz C. (2012) Functional analyses of AGAMOUS family members in Nicotiana benthamiana clarify the evolution of early and late roles of C-function genes in eudicots. Plant Journal 71(6), 990-1001.
Ferrandiz, C., Fourquin, C., Prunet N., SCUTT, C.P., Sundberg, E., Trehin, C., and Vialette-Guiraud A.C. (2010) Carpel Development. Advances in Botanical Research Vol. 55.
Finet, C., Fourquin, C., Vinauger-Douard, M., Dedieu, A., Chambrier, P., Paindavoine, S., and Scutt, C.P. (2010) Parallel structural evolution of Auxin Response Factors in the angiosperms. Plant Journal 63(6), 952-9.
Fourquin, C., Vinauger-Douard, M., Chambrier P, Berne-Dedieu A. and Scutt, C. P. (2007) Functional Conservation between CRABS CLAW Orthologues from Widely Diverged Angiosperms. Annals of Botany 100, 651-657.
Scutt, C.P., Vinauger-Douard M., Fourquin C., Finet C., Dumas, C. (2006). An evolutionary perspective on the regulation of carpel development. J Exp Bot 23, 1-10.
Fourquin, C., Vinauger-Douard, M., Fogliani, B., Dumas, C. and Scutt, C. P. (2005) Evidence that CRABS CLAW and TOUSLED have conserved their roles in carpel development since the ancestor of the extant angiosperms. Proc Natl Acad Sci U S A 102, 4649-4654.
Scutt, CP, Vinauger-Douard M, Fourquin C, Finet C, Dumas C (2005) A portrait of the ancestral flower. Flowering Newsletter 39, 3-8.
Scutt, C. P., Vinauger Douard, M., Fourquin, C., Ailhas, J., Kuno, N., Uchida, K., Gaude, T., Furuya, M. and Dumas, C. (2003) The identification of candidate genes for a reverse genetic analysis of development and function in the Arabidopsis gynoecium. Plant Physiology 132, 653-665.
Hybrid Plant Cell. Patent number: WO/2010/042974A1. Publication date : 22/04/2010.
Easton, A., Fourquin, C., Kawanishi, E. , Nowak, E., Schenk, P. (2009)
Pacific Seeds Pty Ltd, Australie.
Method of Increasing Flower and Fruit Production in a Monocarpic Plant. Balanza V., Berbel A., Ferrándiz C., Fourquin, C., Madueňo F., Martínez-Fernández I. and Serrano Mislata A. In process.