AgreenSkills session, year: 2nd session, 2016
Receiving laboratory: LISBP Engineering of Biological Systems and Processes, Toulouse
Country of origin : Belgium
Enhancing flotation efficiency by functionalizing bubbles with algal cell wall constituents
In the context of climate change and increasing energy needs of the world population, the global interest for sustainable sources to produce energy is growing. One promising resource for biofuel production is microalgae, but their industrial use is limited by the lack of efficient harvesting techniques. Flotation represents a promising harvesting technique that consists in transforming air into bubbles through a solid/liquid suspension. Microalgae get attached to the bubbles produced and are carried out and accumulated on the surface, without being altered. However lack of fundamental research aiming at improving this method makes it for the moment not attractive for industrials. This research project aims at better understanding the biophysics of flotation process. Fundamental knowledge, at the single cell and single molecule level will be acquired and further used to improve algae harvesting by flotation. To achieve this goal we will first deconstruct and analyse the cell wall of the model green algae, Chlorella vulgaris, and evaluate each constituent for its adhesion-aggregation potential using optical tweezers (OT). Then bubbles generated by dissolved air flotation (DAF) will be functionalized with the most promising cell wall constituent and their efficiency for harvesting microalgae will be tested thanks to FluidFM technology. This research project will provide new information on the molecular mechanisms underlying microalgae flotation as well as new ways to improve the efficiency of flotation without damaging the cells and their content.
I studied fundamental microbiology and biotechnologies at the university Paul Sabatier of Toulouse, in France. After obtaining my master degree in 2011, I started a PhD, in 2012, at the Laboratoire d’Analyse et d’Architecture des Systèmes (LAAS-CNRS) in Toulouse. During this PhD, I have worked on important issues related to multidrug resistant microorganisms using nanobiotechnologies, more specifically Atomic Force Microscopy. Starting from there, I chose to do a postdoc in Belgium at the Université catholique de Louvain, between 2015 and 2017, in the nanobiophysics field in which I could learn to lead an original research project using a multidisciplinary approach. Indeed, during this postdoc, I had the opportunity to work on biofilm formation by bacterial pathogens, but more interestingly, I could work on my research project with scientists with different scientific backgrounds. These two research experiences led me to develop research interests in biological interfaces and their interactions with their environment or with other interfaces. To address these challenges, I develop multidisciplinary approaches, at the frontier between biology, chemistry and physics.
1. Fleury OM, McAleer MA, Feuillie C, Formosa-Dague C, Sansevere EA, Bennett D, McLean WHI, Kezic S, Robinson DA, Fallon PG, Foster TJ, Dufrene YF, Irvine AD (2017) Clumping factor B promotes adherence of Staphylococcus aureus to corneocytes in atopic dermatitis. Infect Immun, in press.
2. Feuillie C*, Formosa-Dague C*, Hays MCL*, Vervaeck O, Derclaye S, Brennan M, Foster TJ, Geoghegan J, Dufrêne YF (2017) Biofilm accumulation by the staphylococcal protein SdrC: molecular mechanism and inhibition. Proc Nat Acad Sci USA 114(14):3738-3743. * co-first-author.
3. Schiavone M.*, Formosa-Dague C.*, Elsztein C., Teste M. A., Martin-Yken H., Morais M. A., Dague E., François J. M. 2016. An Atomic Force Microscopy study of yeast response to ethanol stress. Appl Environ Microbiol, 82, 4789-4801, *co-first-author.
4. Formosa-Dague C., Fu Z. H., Feuillie C., Derclaye S., Foster T. J., Geoghegan J. A. Dufrêne Y. F. 2016. Forces between Staphylococcus aureus and human skin. Nanoscale Horizons, 1, 298-303.
5. Solopova A., Formosa-Dague C., Courtin P., Furlan S., Veiga P., Armalyt A., Sadauskas M., Kok J., Hols P., Dufrêne Y. F., Kuipers O. P., Chapot-Chartier M. P., Kulakauskas S. 2016. Regulation of cell wall plasticity by nucleotide metabolism in Lactococcus lactis. J Biol Chem, 294:21, 11323-11336.
6. Smolyakov G., Formosa-Dague C., Severac C., Duval R. E., Dague E. 2016. High speed indentation measures by FV, QI and QNM introduce a new understanding of bionanomechanical experiments. Micron, 85, 8-14.
7. Formosa-Dague C., Feuillie C., Beaussart A., Derclaye S., Kucharikova S., Lasa I., Van Dijck P., Dufrêne Y. F. 2016. Binding forces of the staphylococcal polysaccharide intercellular adhesin. ACS Nano, 10:3, 3443-3452.
8. Pillet F., Formosa-Dague C., Baaziz H., Dague E., Rols M. P. 2016. Cell wall as a target for bacteria inactivation by pulsed electric fields. Sci Rep, 6:19778.
9. Formosa-Dague C., Speziale P., Foster T. J., Geoghegan J. A. Dufrêne Y. F. Zinc-dependent mechanical properties of Staphylococcus aureus biofilm-forming protein SasG. 2016. Proc Nat Acad Sci USA, 113:2, 410-415.
10. Herman-Bausier P., Formosa-Dague C., Feuillie C. Valotteau C., Dufrêne Y. F. 2015. Forces guiding staphylococcal adhesion. J. Struct Biol.
11. Lachaize V., Formosa C., Smolyakov G., Guilbeau-Frugier C., Galés C., Senard J. M., Dague E. Atomic Force Microscopy : an innovative technology to explore cardiomyocyte cell surface in cardiac physio/pathophysiology. 2015. Lett Appl NanoBioSci, 4:4, 321-334.
12. Liu R.*, Formosa C.*, Dagkessamanskaia A., Dague E., François J. M., Martin-Yken H. Combining atomic force microscopy and genetics to investigate the role of knr4 in Saccharomyces cerevisiae sensitivity to k9 killer toxin. 2015. Lett Appl NanoBioSci, 2015, 4:4, 306-315, *co-first author.
13. Formosa C., Herold M. Vidaillac C., Duval R. E., Dague E. Unraveling of a mechanism of resistance to colistin in Klebsiella pneumonia using Atomic Force Microscopy. 2015. J Antimicrob Chemother, 70:8, 2261-70.
14. Formosa C., Pillet F., Schiavone M., Duval R. E., Ressier L., Dague E. 2015. Generating living cells arrays for Atomic Force Microscopy studies. Nat Protoc, 10:1, 199-204.
15. Formosa C., Lachaize V., Galés C., Rols M. P., Martin-Yken H., François J. M., Duval R. E., Dague E. 2015. Mapping HA-tagged protein at the surface of living cells by Atomic Force Microscopy. J Mol Recognit, 28, 1-9.
16. Formosa C., Schiavone M., Boisrame A., Richard M. L., Duval R. E., Dague E. 2015. Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by Atomic Force Microscopy. Nanomedicine NBM, 11, 57-65.
17. Schiavone M., Vax A., Formosa C., Martin-Yken H., Dague E., François J. M. 2014. A combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts. FEMS Yeast Res, 14:6, 933-947.
18. Pillet F., Lemonier S., Schiavone M., Formosa C., Martin-Yken H., François J. M., Dague E. 2014. Uncovering by Atomic Force Microscopy of an original circular structure at the yeast cell surface in response to heat shock. BMC Biol, 12:6.
19. Pillet F., Chopinet L., Formosa C., Dague E. 2014. Atomic Force Microscopy and pharmacology; from microbiology to cancerology. BBA-Gen Subjects, 1840:3, 1028-1050.
20. Beauvais A., Bozza S., Kniemeyer O., Formosa C., Balloy V., Henry C., Roberson R. W., Dague E., Chignard M., Brakhage A. A., Romani L., Latgé J. P. 2013. Deletion of the α1,3 glucan synthase induces a restructuration of the conidial cell wall responsible for the avirulence of Aspergilus fumigatus. Plos Path, 9:11.
21. François J. M., Formosa C., Schiavone M., Pillet F., Martin-Yken H., Dague E. 2013. Use of Atomic Force Microscopy (AFM) to explore cell wall properties and response to stress in the yeast Saccharomyces cerevisiae. Curr Genet, 59, 187-196.
22. Sapet C., Formosa C., Sicard F., Bertosio E., Zelphati O., Laurent N. 2013. 3D-fection: cell transfection within 3D scaffolds and hydrogels. Ther Deliv, 4:6, 673-685.
23. Formosa C., Schiavone M., Martin-Yken H., François J. M., Duval R. E., Dague E. 2013. Nanoscale effects of Caspofungin against two yeasts species; Saccharomyces cerevisiae and Candida albicans. Antimicrob Agents Ch, 57, 3498-3506.
24. Chopinet L.*, Formosa C.*, Rols M. P., Duval R. E., Dague E. 2013. Imaging living cells surface and quantifying its properties at high resolution using AFM in QITM mode, Micron, 48, 26-33, *co-first author.
25. Pillet F., Sanchez A., Formosa C., Séverac M., Trévisiol E., Bouet J. Y., Anton-Leberre V. 2013. Dendrimer functionalization of gold surface improves the measurements of protein-DNA interactions by surface plasmon resonance imaging, Biosens Bioelectronics, 43, 148-154.
26. Formosa C., Grare M., Jauvert E., Coutable A., Regnouf-de-Vains J. B., Mourer M., Duval R. E., Dague E. 2012. Nanoscale analysis of the effects of antibiotics and CX1 on a Pseudomonas aeruginosa multidrug-resistant strain, Sci Rep, 2, 575.
27. Formosa C., Grare M., Duval R. E., Dague E. 2012. Nanoscale effects of antibiotics on P. aeruginosa, Nanomedicine NBM, 8,12-16.
Direction Générale de l’Armement (DGA) PhD award: obtained for presenting the best PhD funded by the DGA.
American Society for Microbiology (ASM) travel grant: to attend the 7th ASM Biofilms conference in Chicago, IL, USA, October 2015.
SANOFI award: from the Académie des Sciences Inscriptions et Belles Lettres de Toulouse, obtained for presenting the best thesis in biology-health with concrete applications.
Pierre Favard Award: from the Société Française de Microscopie (SFµ), obtained for presenting the best PhD thesis in living sciences which principal tool is a microscopy technique.
George McCracken Infectious Disease Fellow Award: from the American Society for Microbiology, travel grant to attend the Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) in Denver, CO, USA, in 2013.