AgreenSkills session, year: 1st session, 2015
Receiving laboratory: BGPI Biology and genetics of plant-pathogen interactions Montpellier
Country of origin : Spain
Characterization of the transmission activation of Cauliflower mosaic virus
Our research team has discovered an unexpected phenomenon: Cauliflower mosaic virus (CaMV) forms precisely at the arrival of the aphid vector specific transmission morphs that dissociate after aphid take-off. Thus, this virus is transmission-competent only during the presence of vectors on the plant and when transmission is possible and can attribute cellular resources to other steps of the infection cycle for the remaining time. This phenomenon was named ‘transmission activation’ (TA), and requires that CaMV recognises via the plant perception systems the presence of aphids.
My project aims to start characterising the molecular and cellular details of CaMV TA, with an emphasis on identification of plant factors involved in the different steps of TA. This will help to establish a basis for understanding TA, aphid recognition and defence, signalling function and whether they are related.
TA is a very rapid process, thus it is likely that rapid posttranscriptional protein modifications regulate TA. I will try to identify proteins with these modifications that can be analysed by mass spectroscopy to reveal their identity and possibly the posttranscriptional modification. Proteins identified will be then validated. A direct role of the candidates will be assessed by infecting the mutants with CaMV and analyzing for transmission activity and phenotype. This will define the plant proteins involved in TA, and possibly their role. If time allows, this aspect will also be tested by monitoring aphid performance and behaviour on correspondent mutant and wild-type plants, either infected by CaMV or not.
I studied Agricultural Engineering in Madrid (Spain) and joined the Institute of Agricultural Sciences (CSIC) in 2010, where I gained my PhD in 2015. The topic of my doctoral studies was the integration of physical, chemical and biological tactics of pest control and associated viruses on horticultural crops. The scope was mainly ecological, and I worked with aphids and whiteflies, and viruses CMV and CABYV on cucurbits and Solanaceae, to develop new strategies to control the spread of pathogens based on the interference with vector population growth, landing, movement and transmission on integrated production systems. During my PhD fellowship, I was awarded with two grants from the Spanish Government to collaborate with Dr. Dylan Gwynn-Jones in Wales and Dr. Piotr Trebicki in Australia, where I worked in the response of insect vectors and plant pathogens to abiotic stresses (UV, CO2) under a climate change scenario. My background is essentially entomology, plant protection, vector biology and virology, which I have recently combined with molecular tools. My interests lie on the implementation of new alternatives to chemical control against the main agents causing major economic losses in vegetable crops. My mid/long-term scientific objectives comprise the study of plant-insect interactions with ecological approaches, always having in mind future global climate scenarios. To achieve this purpose, I propose reducing insecticide inputs for pest control and adopting environment-friendly measures and sustainable plans to enhance the action of natural enemies and pollinators and improve conservation and biodiversity in agricultural lands according to existing European regulations.
Dáder B, Then C, Berthelot E, Ducousso M, Ng JCK, Drucker M. 2017. Insect transmission of plant viruses: Multilayered interactions optimize viral propagation. Insect Science. DOI: 10.1111/1744-7917.12470;
Dáder B, Moreno A, Gwynn-Jones D, Winters A, Fereres A. 2017. Aphid orientation and performance in glasshouses under different UV-A/UV-B radiation regimes. Entomologia Experimentalis et Applicata. DOI: 10.1111/eea.12583;
Trebicki P, Vandegeer R, Bosque-Perez N, Powell K, Dáder B, Freeman A, Yen A, Fitzgerald G, Luck J. 2016. Virus infection mediates the effects of elevated CO2 on plants and vectors. Scientific Reports, 6: 22785;
* Dáder B, Fereres A, Moreno A, Trebicki P. Elevated CO2 impacts bell pepper growth with consequences to Myzus persicae life history, feeding behaviour and virus transmission ability. 2015. Scientific Reports, 6: 19120;
Dáder B, Plaza M, Fereres A, Moreno A. 2015. Flight behaviour of vegetable pests and their natural enemies under different UV-blocking enclosures. Annals of Applied Biology, 167: 116-126;
Dáder B, Legarrea S, Moreno A, Plaza M, Carmo-Sousa M, Amor F, Viñuela E, Fereres A. 2014. Control of insect vectors and plant viruses in protected crops by novel pyrethroid-treated nets. Pest Management Science, 71: 1397-1406;
* Dáder B, Gwynn-Jones D, Moreno A, Winters A, Fereres A. 2014. Impact of UV-A radiation on the performance of aphids and whiteflies and on the leaf chemistry of their host plants. Journal of Photochemistry and Photobiology B: Biology, 138, 307-316;
Dáder B, Legarrea S, Moreno A, Ambrós CM, Fereres A, Viñuela E, Skovmand O, Bosselmann R. 2014. Insecticide-treated nets as a new approach to control vegetable pests in protected crops. Acta Horticulturae, 1015, 103-111;
* Dáder B, Moreno A, Viñuela E, Fereres A. 2012. Spatio-temporal dynamics of viruses are differentially affected by parasitoids depending on the mode of transmission. Viruses, 4, 3069-3089;
* Cambra I, Martínez M, Dáder B, González-Melendi P, Gandullo J, Santamaría ME, Díaz I. 2012. A cathepsin F-like peptidase involved in barley grain protein mobilization, HvPap-1, is modulated by its own propeptide and by cystatins. Journal of Experimental Botany, 63, 4615-4629.
Doctorate with distinction award